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Objective:Observing the effects of HIIT intervention on liver morphology and liver triglycerides in naturally aged rats. Methods: 8 month old SPF rats(n=70)were randomly divided into control group (C) and HIIT group (H). Group H exercised alternately with 70% -90% -50% -70% VO2max intensity for 46 minutes / day, 3 days / week, and lasted 24 weeks. Rat liver triglyceride content was tested and liver sections were analyzed. Results: There was a difference in the level of triglycerides between the baseline level of group H and 24 weeks (P = 0.0000); there was no significant difference between the intrahepatic triglycerides of group H rats and that of group C ( P> 0.05). The liver triglyceride content of rats in groups C and H showed a steady downward trend. Conclusion: The 24-week HIIT training intervention has a positive effect on the recovery of histological morphology in the liver of naturally aged rats; 24 weeks of HIIT training intervention will not affect the liver triglyceride content of naturally aging rats.