Effect of Aerobic Exercise Prescription Combined with Dietary Intervention on MAPK Signal Pathway in Simple Obese Children

Abstract

Purpose: Observe the effect of aerobic exercise prescription combined with diet control on MAPK signaling pathways in children with simple obesity, to explore the molecular mechanisms of weight loss and health promotion in children with simple obesity. Methods: 18 male children with simple obesity were given aerobic exercise training for 4 weeks, 6 days a week, twice a day, each exercise for 2 to 2.5 hours. The dietitian formulated the corresponding diet according to the metabolic rate and weight of each subject, and strictly implemented it. And body composition, blood glucose, blood lipids, serum insulin and other indicators were test before and after the experimental. In addition, the mRNA chip was used to randomly detect the gene expression in the MAPK signaling pathway in the peripheral blood of 5 subjects before and after the experiment. Results: (1) after 4 weeks, the body shape indicators decreased significantly (p<0.05); the blood glucose and lipid metabolism indicators decreased significantly (p<0.05) (2) The gene chip test results found that before and after the experiment, a total of 27 genes in the MAPK signaling pathway were changed. These 27 genes were involved in the four pathways of ERK1/2, ERK5, p38 MAPK, and JNK. Conclusion: (1) 4 weeks of aerobic exercise prescription combined with diet control could significantly reduce the body fat rate of simple obese children, and could significantly improve their glucose and lipid metabolism disorder. (2) 4 weeks of aerobic exercise prescription combined with diet control could play a significant role in MAPK signaling pathway of simple obese children.